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KMID : 1025520090510030185
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Journal of Animal Science and Technology
2009 Volume.51 No. 3 p.185 ~ p.192
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Characterization of the Bovine FASN Gene Variation for Carcass and Beef Quality Traits in Hanwoo
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Li Song-Lan
Kim Sang-Wook
Lee Jung-Jae
Lee Jun-Heon
Yoon Du-Hak
Kim Jong-Joo
Jung Young-Chul
Jeon Soon-Hong
Choi Jae-Won
Kim Nae-Su
Kim Kwan-Suk
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Abstract
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Fatty acid synthase (FASN) is a multi-functional enzyme with a central role in the synthesis of long-chain fatty acid and has been considered as a positional candidate gene for BTA 19 quantitative trait loci (QTL) affecting milk-fat content and fatty acid composition. In this study, we sequenced the FASN gene in several cattle breeds including Hanwoo and imported beef cattle, and identified novel DNA polymorphisms and their linkage relationship in Hanwoo. We found a significant frequency difference of the FASN (AF285607) g.17924 A G polymorphism between Hanwoo (70%) and other breeds and this polymorphism has been known for an association with fatty acid composition in Angus.
Furthermore, by direct DNA sequencing in 18 unrelated Hanwoo, we identified 27 SNPs including nine novel variations in the FASN gene. Among 27 SNPs identified in the FASN gene, four SNPs were further genotyped in 100 Hanwoo and 96 imported beef cattle, and analyzed for haplotype construction and association with beef quality traits. We performed haplotype block and linkage disequilibrium studies using four selected SNPs. Two different haplotype blocks (block A: g.10568 C T and g.11280 GA; block B: g.13125 C T and g.17924 G A) were constructed and the block A in particular had a very high r2 (0.936), which indicated a nearly complete linkage disequilibrium existed between the g.10568 C T and g.11280 G A polymorphisms. A total of four major haplotypes (frequency > 0.05) were identified with the four polymorphisms including TATG (0.36), CGCG (0.31), CGTA (0.19) and TACG (0.06). Statistical association analysis revealed that the g.10568 C T and g.11280 G A polymorphisms in the FASN were significantly associated with meat color (P=0.004) and texture (P=0.0114). The g.13125 C T and g.17924 G A polymorphisms in the FASN were also significantly associated with back-fat thickness and quantity index (P=0.0179 and 0.0495, respectively). Our findings suggested that the FASN gene polymorphisms may be used for determining the (unsaturated) fatty acid contents and carcass trait in the Hanwoo beef.
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KEYWORD
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FASN, SNPs, HRM assay, Hanwoo, Beef quality
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